The movie has an absolute ton of characters, although most of them have extremely brief appearances, particularly in the case of the G.I. Joe side characters. But at least they don't all get killed off within the first 15 minutes am i rite. This list is by no means complete, as several of the G.I. Joes have such brief cameo appearances that getting a decent shot of them is too tricky, but most of the guys are here.
As with everything ever there are far more good characters than bad ones, and whilst the new characters to G.I. Joe mainly get the most screentime, loads of the older ones make an appearance somewhere in the movie.
Alpine is the Joes mountain climbing expert who is seen in the battle at the Statue of Liberty destroy Cobra air units with the help of Snake Eyes. He's later guarding Serpentor's cell with Bazooka and Alpine and is inured by Nemesis Enforcer.
Beachhead is one of the high-up members of the Joe team and in the movie he is first seen in the battle at the Statue of Liberty fighting alongside Iceberg. Later on he is given the task of training the new recruits -Big Lob, Tunnel Rat, Jinx, Chuckles, Law and Falcon - at the G.I. Joe base and they all pass his tests in one way or another. He also takes part in the final battle against Cobra-La.
Big Lob is one of the new characters and a new recruit to G.I. Joe. It seems he used to be a basketball player and he usually always talks like he is a commentator for the sport. He's also quite good with grenades and is shown to use these weapons a couple of times to take out targets.
Chuckles is one of the new characters introduced in the movie but he's the most boring of the lot as he never speaks - why he can't talk is never explained, but he does show that he has great strength and endurance, managing to do things such as pick up large missiles and hurl them at enemy tanks and he also survives a helicopter crash from which he was clinging on the outside of.
Cross Country usually pilots the Joe's Havoc vehicles, and at the start of the movie his Havoc drives off by accident. He only gets one line in the movie but does take part in the final battle against Cobra-La.
Doc is the Joe's main medical officer and sees to Duke after he is injured by Serpentor. He only speaks twice in the movie and both times are off screen, but during his second line he radios Hawk and tells him that Duke has come out of his coma.
Dial-Tone is the communications specialist for the Joes and works with Mainframe to get the B.E.T. to work during the Himalayas test. This is the only notable thing he does in the entire movie.
Duke is the second in command of G.I. Joe and at the start of the movie, stops Cobra from destroying the Statue of Liberty. In the movie it's revealed that he has a half-brother, Falcon, who he risks his life for and almost dies as a result. He is almost killed by Serpentor and goes into a coma, but at the end of the movie it's revealed that he has come out of it. The original idea was for Duke to die in the movie, but this was changed at the last minute to him surviving after all.
Falcon is a new character and is revealed to be Duke's half-brother. He is a real slacker and doesn't take his duty as a soldier seriously, and he is eventually sent to the Slaughter House after neglecting his duties. It's there where he starts to wise up and by the end of the movie he is a real fighter and saves the Earth by using the B.E.T. to destroy the spores before they rain down on the planet. Originally, Falcon was actually supposed to be Hawk's son.
Flint is the third in command of G.I. Joe and is first seen in the movie at the Statue of Liberty battle, managing to knock down several Cobra troops all at once. Later on in the movie he suggests letting himself, Lifeline and Iceberg go to the Himalayas to look for Roadblock's missing unit to Hawk, who lets them. Flint isn't seen again until later in the movie when Roadblock finds them and he later teams his unit up with Falcon's to attack Cobra-La.
Gung-Ho is your average marine and we first see him in the movie at the Statue of Liberty battle. His next and last appearance is when he, Bazooka and Alpine are guarding Serpentor's cell. Gung-Ho is injured by Nemesis Enforcer, but he manages to sound off the alarm first.
Hawk is the commander of the G.I. Joe team and is also one of their best fighter jet pilots. Most of the time he's seen in the movie is just him giving orders out but he later leads a team into Cobra-La, but is captured. He's later freed and takes Slaughter, Jinx and Falcon towards Serpentor's throne room so they can switch off the B.E.T. but he offers to stay behind to fight off some spiders and lets them go and do it. He survives the battle and later tells everyone that Duke has come out of his coma.
Iceberg works as a snow trooper for the Joes, although he's first seen in the movie fighting alongside Beachhead at the Statue of Liberty battle scene. He doesn't appear again for a while due to him being in Flint's unit sent out to find Roadblock, and later teams up with Falcon's unit to invade Cobra-La.
Jinx is another new character, and one of the few female G.I. Joes. She was trained to be a ninja and knows a great deal about martial arts, and she also rejects Falcon when he tries his moves on her, although they seem to be close friends by the end of the movie. She is the one that defeats Pythona in the final battle against Cobra-La.
Lady Jaye is the Joe's covert operations specialist and usually attacks enemies with spears. In the movie she doesn't get to do much as she is part of Roadblock's unit that gets captured early on in the movie. Like everyone else in that unit she escapes later on in the film and fights Cobra-La's forces in the final battle.
Law was a part of the military police but he's joined the G.I. Joe team. He's also got a dog called Order who can sniff out bombs, making him quite useful when it comes to finding explosives.
Lifeline is another medic for the G.I. Joe along with Doc, but he'll also happily take part in a fight if needed. He isn't in the movie much because he's part of Flint's team that go out to look for Roadblock. After they find him they later team up with Falcon's unit and start an assault on Cobra-La.
Lift-Ticket is a chopper pilot for the Joes and he and Wild Bill fly Falcon out after he's been sentenced. He's also the one who simply boots Falcon out of the helicopter. Like most of the other Joes, he also takes part in the final battle.
Low-Light is a sharp-shooter who only gets one line of dialogue in the movie, during the scene where Falcon is put on trial. This isn't his last appearance however as he takes part in the battle against Cobra-La.
Mainframe is the Joe's computer specialist and works on getting the B.E.T. up and running in the Himalayas test site. He doesn't get to do anything important after this but he is at the final battle against Cobra-La.
Mercer used to be a Cobra Viper but he saw what Cobra was doing was wrong and changed sides. He was sent to the Slaughter House to be taught by Sgt. Slaughter in becoming the perfect soldier, along with Taurus and Red Dog. Thanks to him being an ex-Cobra he has some valuable knowledge about their bases and this helps the Renegades gain access to Cobra's Terror Drome. Like most other Joes he is last seen fighting in the final battle against Cobra-La.
Quick Kick used to be a stunt man for movies but he joined the Joes. He's a highly trained martial arts expert, but in the movie he is part of Roadblock's captured unit and doesn't get to do much. He is eventually freed in time to help out everyone else against Cobra-La at the end of the movie.
Red Dog is one of Slaughter's renegades and the movie marks his first appearance in G.I. Joe. He used to be an American football player but was banned from the game for unnecessary roughness and joined the Joes instead.
Roadblock wanted to be a chef but instead joined G.I. Joe, and usually gets to use the heavy weaponry against Cobra. In the movie he leads a unit to hunt down a Cobra squad but is captured by Cobra-La's forces. He later escapes thanks to Cobra Commander and runs into Flint's unit that was sent out to find him. Roadblock then takes everyone back towards the Cobra-La base and takes part in the final battle.
Scarlett is the main female character in the G.I. Joe cartoon, but in the movie she doesn't get a big role. She's first seen punching Torch's lights out at the Statue of Liberty battle and makes appearances in most of the other major battles in the film, including the final battle against Cobra-La.
Shipwreck is first seen in the movie during the Statue of Liberty battle where Bazooka almost accidentally kills him. He's part of Roadblock's captured unit and isn't freed until much later on where he then fights Cobra-La with the other Joes.
Sgt. Slaughter is a special drill instructor for G.I. Joe and currently trains the Renegades - Mercer, Red Dog and Taurus - at the "Slaughter House". He is later given a new recruit to train up when Falcon is dropped off and he makes him go through the Slaughter House's assault course. Together, they later attack Cobra's Terror Drome and blow it up, and Slaughter is at the last major battle of the movie where he fights and defeats Nemesis Enforcer.
Snake Eyes may be a popular character with fans but he gets to do very little in the movie. He's shown in the opening battle a couple of times, but he's also part of Roadblock's unit which gets captured when they enter Cobra-La. Whilst he does eventually escape, he isn't shown to do anything cool in the final battle. As usual, he never speaks either.
Snow Job is an arctic trooper for the Joes along with Iceberg, and in the movie he's part of Roadblock's unit which gets captured by Cobra-La forces not that long into the movie. He and the rest of the unit do eventually escape much later on to fight Cobra-La. So he doesn't get to do much but everyone really remembers him for his PSA anyway.
Taurus used to work at a circus but he left to join G.I. Joe. He's been put into Sgt. Slaughter's Renegades unit along with Red Dog and Mercer, and is is often seen with a large sword. He's also not that bright but he makes up for it with his sheer strength.
Tunnel Rat is yet another new character who joins the G.I. Joe force along with Big Lob, Jinx, Falcon, Law and Chuckles. He is first put onto an assault course by Beachhead but he gets through it easily by crawling though a drain pipe to get across from one side to the other in no time at all. Later he infiltrates Cobra-La with the other new characters and Flint's unit.
Wild Bill is a helicopter pilot for the Joes, and in the movie he and Lift-Ticket fly Falcon out to the Slaughter House. Lift-Ticket simply boots Falcon out of Wild Bill's copter.
Airtight: Appears during the final battle scene.
Barbeque: The guy with one of the best PSAs makes an appearance at the Statue of Liberty and final battle scenes.
Bazooka: Appears at the Statue of Liberty battle and is later injured by Nemesis Enforcer at the scene where Serpentor is freed.
Blowtorch: Makes a few cameos and is at the final battle scene. Everyone remembers him for his PSA though.
Cover Girl: Appears during the Statue of Liberty battle, the scene where Cobra get the B.E.T. and the final battle scenes.
Crankcase: Appears in the scene where Cobra get the B.E.T. and the final battle scenes.
Dusty: Is part of Roadblock's unit that gets captured, but unlike everyone else in said unit he doesn't get to say anything.
Recondo: Takes part in most of the major battles in the movie.
Rock 'n Roll: Also takes part in most of the major battles.
Sci-Fi: Is present at the scene where Cobra get the B.E.T. and is also at the final battle.
Spirit: Is at the opening and last battle scenes.
Stalker: Is present at the final battle against Cobra-La.
COBRA and COBRA-LA
The main bad guys of the previous seasons of the cartoon have mainly minor roles here too with the likes of Golobulus having the limelight.
Baroness continues to serve under Serpentor and is shown as being loyal to him a couple of times in the movie, although Cobra Commander doesn't approve. She's also the one who finds out where the Joes are hiding the B.E.T. by disguising herself as a worker. She is also in the final battle scene and survives it, given that she later appears in the next series of G.I. Joe.
Buzzer is one of the Dreadnoks and is easily identified as he's always got a pair of glasses on. He is often seen wielding a small chainsaw throughout his appearances in the movie.
Cobra Commander used to be the leader of the Cobra force but he was replaced by Serpentor in the second season of the cartoon. This has led to him developing a deep hatred for Serpentor and he tries to dispose of him whenever an opportunity arrives. In the movie Cobra Commander is bought before Golobulus who punishes him for failing to conquer the Earth by mutating him into a snake. His snake form is last seen in the movie attacking one of Serpentor's snakes, but he does survive as he'll be back in the next season.
Destro makes weapons for Cobra and, after getting fed up of Cobra Commander's constant failures, helped Dr. Mindbender create Serpentor. In the movie he only has a minor role and takes part in most of the battles, including the last one. It's not shown what happened to him but he must have survived it as he appears in the next season.
Golobulus once ruled the Earth but he and his people were forced into hiding with the coming of the ice-age. 40,000 years later he sent Cobra Commander to reclaim the Earth but he failed to do so and now he wants to do it himself with the use of his mutation spores. As well as this he reveals that he was the one that created Serpentor. Golobulus fights Falcon at the end of the movie and has one of his eyes stabbed, but he flies away as the spores explode. However he is never seen again in the animated series so most fans believe that he died when Cobra-La exploded.
Dr. Mindbender is the chief scientist for Cobra and was the one that created Serpentor. But it's revealed that Golobulus was actually the true one who created him from behind the scenes and that Dr. Mindbender was being subliminally told what to do. Dr. Mindbender is present at the final battle is not seen again in the next series, so it's likely he died when Cobra-La was destroyed.
Nemesis Enforcer is a new character and one of Cobra-La's toughest soldiers, and the only one that looks mostly human, apart from the giant wings he has on his back. He displays great a great amount of strength throughout the movie, although he never speaks and only grunts. He is eventually defeated by Sgt. Slaughter and killed when thrown down into a spike pit. Surely he has one of the best names ever though.
Pythona is a new character who appears early on in the movie to show Serpentor the B.E.T. and how he must get it to help in his quest to take over the world. She is part of Cobra-La and goes on to make several more appearances in the movie, but she never gets to do any actual fighting again until the final battle where she faces Jinx. She tries to push Jinx into a spike pit but misses and ends up falling into it herself.
Ripper is one of the Dreadnoks and he appears in most of the battle scenes.
Serpentor assumed leadership of Cobra at the end of the second season of G.I. Joe and replaced Cobra Commander, who wasn't very happy about it. In the movie it's revealed that Serpentor was actually created by Golobulus, who he meets and the two of them join together to take over the world. He nearly kills Duke and later on fights Falcon but a malfunction in his hover board leads to him going out of control and he isn't seen in the movie again, but he will be back for the next season.
Thrasher is one of the Dreadnoks and in the movie he doesn't get to do much other than take part in a few of the battle scenes.
Tomax is Xamot's brother, and he participates in all of the major battles in the movie with his sibling, but other than that he doesn't have much else to do, getting to say very little.
Torch is another Dreadnok and is usually seen using a flamethrower. He first appears in the movie at the Statue of Liberty battle where he is punched in the face by Scarlett, but he isn't captured and goes on to appear in most of the battle scenes in the rest of the movie.
Xamot is Tomax's brother, and he participates in all of the major battles in the movie with his sibling, but other than that he doesn't have much else to do, getting to say very little. Xamot looks exactly the same as Tomax, but he has a scar on his right cheek which Tomax does not have.
Zandar is Zartan's brother and is another one of the Dreadnoks. Like most of the other Dreadnoks he only has a minor role in the movie and is in most of the battle scenes.
Zarana is Zartan's sister and is shown to be a master of disguise. She manages to get Falcon to take her around the G.I. Joe base where she learns all of it's security secrets. After this she is seen in most of the movie's fight scenes.
Zartan is the leader of the Dreadnoks, and he is shown to be someone who can easily be bribed. When Serpentor is captured he doesn't want to go and rescue him but is persuaded when Pythona offers him a large jewel for a reward. Zartan makes appearances in most of the movies fight scenes.
Major Bludd: First seen at the opening battle sequence where he is shot down by Duke, but he comes back again a little later on in the movie.
Firefly: Appears in the scene where Cobra tries to take the B.E.T. in the Himalayas.
Monkeywrench: He's another Dreadnok who appears several times but never says anything so cameo status for him.
Scrap-Iron: Appears in the scene where everyone tells Cobra Commander how terrible he is.
Storm Shadow: Is in the same scene as Scrap-Iron.